episomal vectors encoding reprogramming factors pclxe-h oct4 Search Results


episomal vectors pclxe-h oct4 / p53  (Addgene inc)
90
Addgene inc episomal vectors pclxe-h oct4 / p53
Generation of integration-free Epi-iPSCs from fibroblasts by using episomal vectors. (A) A scheme depicting the procedure. (B) The morphology of Epi-iPSCs and alkaline phosphatase staining on day 14 after transfection with 0–21 μg of each episomal vectors ( OCT4 / <t>p53</t> ; O/P53, SOX2 / KLF4 ; S/K, L-MYC / LIN28A ; M/L), and established Epi-iPSCs as assessed by bright-field microscopy. Scale bar = 100 μm. (C) Number of Epi-iPSCs colonies on day 14 after transfection with different amounts of episomal vectors. The data were represented mean ± SEM and analyzed by one-way ANOVA. *** p < 0.001 ( n = 3).
Episomal Vectors Pclxe H Oct4 / P53, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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episomal vectors encoding reprogramming factors pclxe-h oct4 / shp53  (Addgene inc)
90
Addgene inc episomal vectors encoding reprogramming factors pclxe-h oct4 / shp53
Generation of integration-free Epi-iPSCs from fibroblasts by using episomal vectors. (A) A scheme depicting the procedure. (B) The morphology of Epi-iPSCs and alkaline phosphatase staining on day 14 after transfection with 0–21 μg of each episomal vectors ( OCT4 / <t>p53</t> ; O/P53, SOX2 / KLF4 ; S/K, L-MYC / LIN28A ; M/L), and established Epi-iPSCs as assessed by bright-field microscopy. Scale bar = 100 μm. (C) Number of Epi-iPSCs colonies on day 14 after transfection with different amounts of episomal vectors. The data were represented mean ± SEM and analyzed by one-way ANOVA. *** p < 0.001 ( n = 3).
Episomal Vectors Encoding Reprogramming Factors Pclxe H Oct4 / Shp53, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/episomal vectors encoding reprogramming factors pclxe-h oct4 / shp53/product/Addgene inc
Average 90 stars, based on 1 article reviews
episomal vectors encoding reprogramming factors pclxe-h oct4 / shp53 - by Bioz Stars, 2026-02
90/100 stars
  Buy from Supplier

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Generation of integration-free Epi-iPSCs from fibroblasts by using episomal vectors. (A) A scheme depicting the procedure. (B) The morphology of Epi-iPSCs and alkaline phosphatase staining on day 14 after transfection with 0–21 μg of each episomal vectors ( OCT4 / p53 ; O/P53, SOX2 / KLF4 ; S/K, L-MYC / LIN28A ; M/L), and established Epi-iPSCs as assessed by bright-field microscopy. Scale bar = 100 μm. (C) Number of Epi-iPSCs colonies on day 14 after transfection with different amounts of episomal vectors. The data were represented mean ± SEM and analyzed by one-way ANOVA. *** p < 0.001 ( n = 3).

Journal: Animal Cells and Systems

Article Title: Optimization of episomal reprogramming for generation of human induced pluripotent stem cells from fibroblasts

doi: 10.1080/19768354.2018.1451367

Figure Lengend Snippet: Generation of integration-free Epi-iPSCs from fibroblasts by using episomal vectors. (A) A scheme depicting the procedure. (B) The morphology of Epi-iPSCs and alkaline phosphatase staining on day 14 after transfection with 0–21 μg of each episomal vectors ( OCT4 / p53 ; O/P53, SOX2 / KLF4 ; S/K, L-MYC / LIN28A ; M/L), and established Epi-iPSCs as assessed by bright-field microscopy. Scale bar = 100 μm. (C) Number of Epi-iPSCs colonies on day 14 after transfection with different amounts of episomal vectors. The data were represented mean ± SEM and analyzed by one-way ANOVA. *** p < 0.001 ( n = 3).

Article Snippet: Episomal vectors pCLXE-h OCT4 / p53 , pCLXE-h SOX2 / KLF4 , pCLXE-h L-MYC / LIN28A used for this study were originally described by (Okita et al. ) and were obtained from Addgene (Cat 27078, Cat 27080, and Cat 27077, respectively).

Techniques: Staining, Transfection, Microscopy